The aim of present study is to isolate and characterize bioactive compound from Roscoea procera rhizomes. Roscoea procera rhizomes were extracted with ethanol (95%). This extract was studied phytochemically and bioactive compound is isolated by using thin layer and column chromatography technique. Compound was subjected to spectroscopic analysis such as FT-IR and 1H-NMR. Rf values of extract and isolated bioactive compound was found 0.042, 0.084, 0.136, 0.473, 0.600, 0.663, 0.736 and 0.67 respectively. Chromatographically pure compound was isolated by this method. It can be indicated that, isolated compound was found to be steroid or steroidal-glycoside and it was confirmed by spectroscopic analysis. As far as our knowledge goes this bioactive compound is novel and not reported in Roscoea procera rhizomes yet, which can provides deeper insights into indigenous method of application and effectiveness of plant derivatives in treating different ailments.
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